2BWY

Glu383Ala Escherichia coli Aminopeptidase P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200H
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-05-31
Detector	MARRESEARCH
Spacegroup name	I 41 2 2
Unit cell lengths	138.578, 138.578, 231.389
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	119.520 - 2.400
R-factor	0.174
Rwork	0.172
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bhc
RMSD bond length	0.010
RMSD bond angle	1.178
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.110	0.550
Number of reflections	41368
<I/σ(I)>	13.9	2.5
Completeness [%]	94.1	91.3
Redundancy	5.9	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	SITTING DROP VAPOUR DIFFUSION AT 4C. 2 UL 17 MG/ML APPRO PLUS 2 UL RESERVOIR SOLUTION: 30% MPD, 0.1 M CITRATE PH 7.5, 0.2 M MGACETATE. SOAKED IN RESERVOIR SOLUTION SUPPLEMENTED WITH 1 MM MNCL2 FOR 45 MIN AT 4C PRIOR TO CRYOCOOLING.