2BWR
Crystal Structure of Psathyrella Velutina Lectin at 1.5A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.828, 142.219, 52.161 |
| Unit cell angles | 90.00, 92.10, 90.00 |
Refinement procedure
| Resolution | 52.130 - 1.500 |
| R-factor | 0.159 |
| Rwork | 0.158 |
| R-free | 0.18200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bwm |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.655 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.150 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.070 | 0.260 |
| Number of reflections | 115491 | |
| <I/σ(I)> | 5.3 | 3.3 |
| Completeness [%] | 96.3 | 90.1 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 2.2M (NH4)2SO4,0.1M MES PH 6.5 |
