2BWK

Murine angiogenin, sulphate complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	293
Detector technology	CCD
Collection date	1999-09-20
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	63.762, 56.266, 35.343
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.180 - 1.500
R-factor	0.179
Rwork	0.178
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1agi
RMSD bond length	0.015
RMSD bond angle	1.616
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.070	0.220
Number of reflections	20302
<I/σ(I)>	17	5
Completeness [%]	96.3	83.4
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5		25% PEG 4000, 0.2M (NH4)2SO4, UNBUFFERED, pH 4.50