2BW4
Atomic Resolution Structure of Resting State of the Achromobacter cycloclastes Cu Nitrite Reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 95.414, 95.414, 95.414 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.480 - 0.900 |
| R-factor | 0.118 |
| Rwork | 0.117 |
| R-free | 0.13400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nif |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.167 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.500 | 0.920 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Rmerge | 0.060 | 0.550 |
| Number of reflections | 208276 | |
| <I/σ(I)> | 24 | 2 |
| Completeness [%] | 98.1 | 85.9 |
| Redundancy | 5.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 1.6M AMMONIUM SULPHATE, 100MM SODIUM ACETATE, PH 4.75 |
