2BW3
Three-dimensional structure of the Hermes DNA transposase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-23 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.353, 84.974, 73.856 |
| Unit cell angles | 90.00, 93.71, 90.00 |
Refinement procedure
| Resolution | 29.030 - 2.000 |
| Rwork | 0.185 |
| R-free | 0.21100 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNX (2002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.170 |
| Number of reflections | 48443 | |
| <I/σ(I)> | 14.5 | 7.5 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 3.85 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | pH 6.60 |
