2BVU
D83R mutant of Asaris suum major sperm protein (MSP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Collection date | 2003-09-18 |
| Detector | ADSC |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 54.805, 54.805, 453.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.23 |
| Rwork | 0.226 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1grw |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.505 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.340 |
| Number of reflections | 17093 | |
| <I/σ(I)> | 19.9 | 4.7 |
| Completeness [%] | 93.3 | 78.5 |
| Redundancy | 7.7 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PROTEIN 5 MG/ML; 15% PEG4000; 0.1M AMMONIUM ACETATE (PH 5.9); 2% AMMONIUM SULPHATE; 10 MM DTT |
