2BVU

D83R mutant of Asaris suum major sperm protein (MSP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Collection date	2003-09-18
Detector	ADSC
Spacegroup name	P 41 21 2
Unit cell lengths	54.805, 54.805, 453.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.23
Rwork	0.226
R-free	0.29100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1grw
RMSD bond length	0.012
RMSD bond angle	1.505
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.070	0.340
Number of reflections	17093
<I/σ(I)>	19.9	4.7
Completeness [%]	93.3	78.5
Redundancy	7.7	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				PROTEIN 5 MG/ML; 15% PEG4000; 0.1M AMMONIUM ACETATE (PH 5.9); 2% AMMONIUM SULPHATE; 10 MM DTT