2BVE
Structure of the N-terminal of Sialoadhesin in complex with 2-Phenyl- Prop5Ac
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 104 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.974, 70.961, 102.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.720 - 2.200 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qfo |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.260 |
| High resolution limit [Å] | 2.100 | 2.170 |
| Rmerge | 0.130 | 0.340 |
| Number of reflections | 13244 | |
| <I/σ(I)> | 10.45 | 2.3 |
| Completeness [%] | 92.0 | 92 |
| Redundancy | 4.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 1:1 DROP WITH 10MG/ML PROTEIN, 10MM DTT AND 24% PEG4000, 0.16MM MGCL2, 0.08 TRIS PH 8.5, 25MM COMPOUND |
