2BV3
Crystal structure of a mutant elongation factor G trapped with a GTP analogue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-16 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.990, 103.340, 118.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.430 - 2.500 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bm0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.367 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.900 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.440 |
| Number of reflections | 30656 | |
| <I/σ(I)> | 11.5 | 2.7 |
| Completeness [%] | 96.5 | 94.6 |
| Redundancy | 7.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.3 | 17 % PEG8000, 10 MM MGCL2, 100 MM GDPNP, 46 MM TRIS, 100 MM HEPES, pH 7.30 |
