2BV2
beta gamma crystallin from Ciona Intestinalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-28 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.037, 29.788, 57.278 |
| Unit cell angles | 90.00, 121.48, 90.00 |
Refinement procedure
| Resolution | 17.010 - 1.550 |
| R-factor | 0.173 |
| Rwork | 0.169 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1HA4 1hk0 1oki C-TERMINAL DOMAINS SUPERIMPOSED |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.239 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.130 | 0.310 |
| Number of reflections | 21220 | |
| <I/σ(I)> | 2.6 | 1.8 |
| Completeness [%] | 93.0 | 87.7 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.2M AMMONIUM SULPHATE, 0.1M SODIUM ACETATE PH4.6, 30PERCENT PEGMME 2000, WITH ADDITION OF CALCIUM ACETATE, pH 4.60 |
