2BV1
Regulator of G-protein Signalling 1 (Human)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-26 |
| Detector | RIGAKU RAXIS HTC |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.738, 43.202, 58.651 |
| Unit cell angles | 78.43, 85.30, 69.74 |
Refinement procedure
| Resolution | 57.450 - 2.000 |
| R-factor | 0.191 |
| Rwork | 0.188 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1AGR AND 1FQJ |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.534 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.030 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.340 |
| Number of reflections | 22497 | |
| <I/σ(I)> | 16.3 | 2.7 |
| Completeness [%] | 90.6 | 65.7 |
| Redundancy | 3.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 4.1M SODIUM FORMATE, 3% GLYCEROL. 1:1 MIXTURE WITH RGS1 PROTEIN AT 23MG/ML., pH 8.00 |
