2BUP
T13G Mutant of the ATPASE fragment of Bovine HSC70
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 100 |
Detector | MACSCIENCE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 143.350, 64.300, 46.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.700 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.22400 |
Structure solution method | OTHER |
RMSD bond length | 0.009 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.058 | 0.140 * |
Total number of observations | 167988 * | |
Number of reflections | 46807 | |
Completeness [%] | 97.4 | 89.5 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 9 | 4 * | pH 9.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | MgATP | 1 (mM) | |
2 | 1 | reservoir | CHES | 50 (mM) | |
3 | 1 | reservoir | 1 (M) | ||
4 | 1 | reservoir | PEG8000 | 20 (%) |