2BUP

T13G Mutant of the ATPASE fragment of Bovine HSC70

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Detector	MACSCIENCE
Spacegroup name	P 21 21 21
Unit cell lengths	143.350, 64.300, 46.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.700
R-factor	0.195
Rwork	0.195
R-free	0.22400
Structure solution method	OTHER
RMSD bond length	0.009
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.058	0.140 *
Total number of observations	167988 *
Number of reflections	46807
Completeness [%]	97.4	89.5
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	9	4 *	pH 9.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MgATP	1 (mM)
2	1	reservoir	CHES	50 (mM)
3	1	reservoir		1 (M)
4	1	reservoir	PEG8000	20 (%)