Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.860, 98.780, 162.900 |
| Unit cell angles | 91.49, 92.03, 107.56 |
Refinement procedure
| Resolution | 18.000 - 2.950 |
| R-factor | 0.2491 |
| Rwork | 0.249 |
| R-free | 0.26680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gs5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.968 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.000 | 3.100 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.050 | 0.360 |
| Number of reflections | 86766 | |
| <I/σ(I)> | 5.4 | 2 |
| Completeness [%] | 96.6 | 94.2 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | CRYSTALS WERE OBTAINED AT 277 K IN ABOUT TWO WEEKS USING THE HANGING DROP TECHNIQUE AND UTILIZING A 10 MG/ML. PROTEIN SOLUTION CONTAINING 20 MM HEPES PH 7.5, 1 MM DITHIOERYTHRITOL, 10% GLYCEROL, 30 MM MAGNESIUM CHLORIDE, 20 MM N-ACETYL-L-GLUTAMATE, 10 MM ADP AND 0.02% SODIUM AZIDE, AND A RESERVOIR SOLUTION WITH 0.1 M SODIUM CACODYLATE PH 6.5, 150-170 MM MAGNESIUM ACETATE AND POLYETHYLENE GLYCOL 8000 |
