2BTV
ATOMIC MODEL FOR BLUETONGUE VIRUS (BTV) CORE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 281 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 795.600, 821.800, 753.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 3.500 |
| R-factor | 0.266 |
| Rwork | 0.266 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.430 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 4.000 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.229 | 0.677 * |
| Total number of observations | 3299866 * | |
| Number of reflections | 21510811 * | |
| <I/σ(I)> | 2.9 | 0.72 |
| Completeness [%] | 53.5 * | 78 * |
| Redundancy | 6.5 | 1.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8 | Burroughs, J.N., (1995) Virology, 210, 217. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 11-16 (%sat) | |
| 2 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 3 | 1 | reservoir | ethylene glycol | 15 (%) | |
| 4 | 1 | drop | protein | 3 (mg/ml) |
