2BTV

ATOMIC MODEL FOR BLUETONGUE VIRUS (BTV) CORE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	281
Detector technology	IMAGE PLATE
Detector	MAR scanner 300 mm plate
Spacegroup name	P 21 21 2
Unit cell lengths	795.600, 821.800, 753.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 3.500
R-factor	0.266
Rwork	0.266
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	24.430 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	4.000
High resolution limit [Å]	3.500	3.500
Rmerge	0.229	0.677 *
Total number of observations	3299866 *
Number of reflections	21510811 *
<I/σ(I)>	2.9	0.72
Completeness [%]	53.5 *	78 *
Redundancy	6.5	1.06

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8		Burroughs, J.N., (1995) Virology, 210, 217. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	11-16 (%sat)
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	reservoir	ethylene glycol	15 (%)
4	1	drop	protein	3 (mg/ml)