2BT9
Lectin from Ralstonia solanacearum complexed with Me-fucoside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-31 |
Detector | ADSC CCD |
Spacegroup name | P 31 |
Unit cell lengths | 43.985, 43.985, 103.076 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 0.940 |
R-free | 0.12010 |
Structure solution method | OTHER |
RMSD bond length | 0.014 |
RMSD bond angle | 0.030 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.670 | 0.960 |
High resolution limit [Å] | 0.940 | 0.940 |
Rmerge | 0.063 | 0.380 |
Number of reflections | 142705 | |
<I/σ(I)> | 15.32 | 1.28 |
Completeness [%] | 98.3 | 79.5 |
Redundancy | 5.79 | 3.68 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | HANGING DROPS WITH 2.5 UL RSL AT 15MG/ML, 2 UL OF RESERVOIR SOLUTION (30% PEG 6000, 0.1M TRIS/HCL PH 8.2) AND 1 UL OF GLYCINE. |