2BT2
Structure of the regulator of G-protein signaling 16
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-23 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 160.585, 31.743, 151.766 |
Unit cell angles | 90.00, 94.39, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.195 |
Rwork | 0.192 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1agr |
RMSD bond length | 0.015 |
RMSD bond angle | 1.404 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.230 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.080 | 0.450 |
Number of reflections | 216171 | |
<I/σ(I)> | 9.5 | 2.39 |
Completeness [%] | 95.6 | 85.2 |
Redundancy | 3.32 | 2.66 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 0.1 M BIS-TRIS, PH 5.5, 26% PEG3350, 0.1 M NH4AC |