2BSZ
Structure of Mesorhizobium loti arylamine N-acetyltransferase 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-15 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.239, 97.304, 114.346 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 74.540 - 2.000 |
R-factor | 0.198 |
Rwork | 0.195 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gx3 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.457 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.080 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.100 | 0.250 |
Number of reflections | 40315 | |
<I/σ(I)> | 4.85 | |
Completeness [%] | 98.5 | 91.3 |
Redundancy | 4.6 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | pH 7.50 |