2BSX
Crystal structure of the Plasmodium falciparum purine nucleoside phosphorylase complexed with inosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 95.117, 95.117, 47.075 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.900 - 2.000 |
| R-factor | 0.268 |
| Rwork | 0.264 |
| R-free | 0.34200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lx7 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.931 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.350 |
| Number of reflections | 27984 | |
| <I/σ(I)> | 10 | 5 |
| Completeness [%] | 87.0 | 80 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 10 % PEG 8000, 0.1 M HEPES PH 7.5, 30 % HEXANETRIOL |
