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2BSW

Crystal structure of a glyphosate-N-acetyltransferase obtained by DNA shuffling.

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 5.0.2
Synchrotron siteALS
Beamline5.0.2
Temperature [K]100
Detector technologyCCD
Collection date2002-12-12
DetectorADSC CCD
Spacegroup nameC 1 2 1
Unit cell lengths69.323, 49.393, 46.492
Unit cell angles90.00, 103.47, 90.00
Refinement procedure
Resolution45.170 - 1.630
R-factor0.181
Rwork0.179
R-free0.21300
Structure solution methodSAD
RMSD bond length0.010
RMSD bond angle1.360
Data reduction softwareMOSFLM
Data scaling softwareSCALEPACK
Phasing softwareCCP4
Refinement softwareREFMAC (5.2.0005)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.690
High resolution limit [Å]1.6301.630
Rmerge0.0700.160
Number of reflections18939
<I/σ(I)>20.28
Completeness [%]97.498.1
Redundancy2.94
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
14.6100 MM SODIUM ACETATE, PH 4.6 250 MM AMMONIUM SULFATE 25% PEG 4000

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