2BSK

Crystal structure of the TIM9 Tim10 hexameric complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-05
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	107.430, 107.430, 110.588
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.000 - 3.300
R-factor	0.2679
Rwork	0.268
R-free	0.31930
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.440
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	RESOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.600
High resolution limit [Å]	3.450	3.450
Rmerge	0.080	0.370
Number of reflections	9357
<I/σ(I)>	17	2.42
Completeness [%]	89.9	85
Redundancy	8.8	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1 M TRIS-HCL PH 8.5, 28% W/V PEG 3000, 0.2 M NACL