2BSI
Crystal structure of the type III secretion chaperone SycT from Yersinia enterocolitica (crystal form 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.539, 63.090, 74.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.110 - 2.010 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bsh |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.071 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.100 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.370 |
| Number of reflections | 16331 | |
| <I/σ(I)> | 12.3 | 4.6 |
| Completeness [%] | 98.4 | 96.4 |
| Redundancy | 7.8 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1.6 M AMMONIUM SULFATE, 0.1 M CAPS PH 10.5, 150 MM LITHIUM SULFATE HANGING-DROP VAPOR DIFFUSION AT 4 DEG C |
