2BSE

Structure of Lactococcal Bacteriophage p2 Receptor Binding Protein in complex with a llama VHH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	74.763, 83.939, 137.793
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.700
R-factor	0.253
Rwork	0.251
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bsd
RMSD bond length	0.006
RMSD bond angle	0.883
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.770
High resolution limit [Å]	2.700	2.700
Rmerge	0.100	0.300
Number of reflections	23055
<I/σ(I)>	5.6	2
Completeness [%]	99.0
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		pH 6.50