2BSD

Structure of Lactococcal Bacteriophage p2 Receptor Binding Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	76.108, 96.250, 149.491
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.221
Rwork	0.220
R-free	0.25200
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.148
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE/RESOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.090	0.330
Number of reflections	48171
<I/σ(I)>	6.7	2
Completeness [%]	99.0
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		A VOLUME OF 1 MICROL OF PROTEIN 6 TO 9 MG/ML) WAS MIXED WITH 1 MICROL OF RESERVOIR SOLUTION CONTAINING 0.85-1.1 M AMMONIUM SULPHATE IN 0.1 M MES PH 6.5. CRYSTALS WERE IMPROVED BY MICROSEEDING.