2BSA

Ferredoxin-Nadp Reductase (Mutation: Y 303 S) complexed with NADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2004-12-03
Detector	MAR scanner 345 mm plate
Spacegroup name	P 65
Unit cell lengths	87.154, 87.154, 96.815
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.370 - 1.920
R-factor	0.197
Rwork	0.197
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gjr
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.320	2.020
High resolution limit [Å]	1.920	1.920
Rmerge	0.070	0.070
Number of reflections	31825
<I/σ(I)>	23	8.6
Completeness [%]	99.7	98
Redundancy	12.1	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		pH 5.50