2BS7
Crystal structure of F17b-G in complex with chitobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 62 |
| Unit cell lengths | 86.698, 86.698, 57.043 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.510 - 2.100 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o9w |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.040 | 0.410 |
| Number of reflections | 8000 | |
| <I/σ(I)> | 29 | 5.5 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 9.3 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | HANGING DROP: 1 MICROLITER OF 1.2 M LI2SO4, 10 MM NICL2, 100 MM TRIS PH 8.5 PLUS 1 MICROLITER 16 MG/ML F17BG |
