2BS6
LECTIN FROM RALSTONIA SOLANACEARUM COMPLEXED WITH XYLOGLUCAN FRAGMENT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-29 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 64.127, 64.127, 128.121 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.150 - 1.800 |
| R-factor | 0.148 |
| Rwork | 0.146 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | COMPLEX WITH SELENIO SUGAR (WATER LIGAND REMOVED |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.833 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.150 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.110 | 0.470 |
| Number of reflections | 24221 | |
| <I/σ(I)> | 14.9 | 3.1 |
| Completeness [%] | 99.7 | 96.7 |
| Redundancy | 8.35 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | RSL/FUCOSE CRYSTALS WERE PREPARED BY HANGING DROPS METHODS USING 1.5M NH42(S04) AND SOAKED IN 2.7 MG/ML XXFG SOLUTION |
