2BS4
GLU C180 -> ILE VARIANT QUINOL:FUMARATE REDUCTASE FROMWOLINELLA SUCCINOGENES
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 277 |
Detector technology | CCD |
Collection date | 2001-02-26 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.104, 188.766, 117.817 |
Unit cell angles | 90.00, 104.47, 90.00 |
Refinement procedure
Resolution | 29.650 - 2.760 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bs2 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.710 | 2.830 |
High resolution limit [Å] | 2.760 | 2.760 |
Rmerge | 0.100 | 0.360 |
Number of reflections | 79393 | |
<I/σ(I)> | 6 | 1.2 |
Completeness [%] | 87.3 | 82.9 |
Redundancy | 2.2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | pH 6.00 |