2BRA
Structure of N-Terminal FAD Binding motif of mouse MICAL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.969, 87.338, 80.810 |
| Unit cell angles | 90.00, 111.68, 90.00 |
Refinement procedure
| Resolution | 27.940 - 2.000 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.26600 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.976 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.590 |
| Number of reflections | 62811 | |
| <I/σ(I)> | 15.2 | 1.56 |
| Completeness [%] | 98.9 | 94 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | PROTEIN SAMPLE: 20 MM HEPES PH 7.0, 1M NACL, 1MM DTT, 4 MG/ML MICAL RECERVOIR: 100MM NA ACETATE PH 4.6, 42% W/V PEG 2K MME 0.3 M (NH4)2SO4 |
