2BR4
cmcI-D160 Mg-SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.541, 102.364, 181.905 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.300 - 2.600 * |
| R-factor | 0.202 |
| Rwork | 0.199 |
| R-free | 0.25900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bm8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.140 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.300 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.099 * | 0.490 |
| Total number of observations | 876420 * | |
| Number of reflections | 53933 | |
| <I/σ(I)> | 13.4 | 1.9 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 7. * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 20 * | * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 0.2 (M) | ||
| 2 | 1 | reservoir | Tris | 0.1 (M) | pH7.5 |
| 3 | 1 | reservoir | PEG4000 | 10-14 (%) | |
| 4 | 1 | reservoir | PEG1000 | 4-7 (%) | |
| 5 | 1 | drop | SAM | 10 (mM) |
