2BQX
Inorganic Pyrophosphatase from the Pathogenic Bacterium Helicobacter pylori-Kinetic and Structural Properties
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL12B2 |
Synchrotron site | SPring-8 |
Beamline | BL12B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-12 |
Detector | ADSC CCD |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 102.650, 102.650, 93.630 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.930 - 1.900 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qez |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.930 | 2.020 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.100 | 0.360 |
Number of reflections | 192336 | |
Completeness [%] | 99.4 | 99.4 |
Redundancy | 8.1 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |