2BQ5
MS2 (N87AE89K mutant) - RNA hairpin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-01 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 467.050, 285.590, 273.300 |
| Unit cell angles | 90.00, 122.23, 90.00 |
Refinement procedure
| Resolution | 29.980 - 2.910 |
| R-factor | 0.293 |
| Rwork | 0.293 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.200 | 0.500 |
| Number of reflections | 221184 | |
| <I/σ(I)> | 7.2 | 1.4 |
| Completeness [%] | 33.0 | 7 |
| Redundancy | 1.5 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
