2BQ1
Ribonucleotide reductase class 1b holocomplex R1E,R2F from Salmonella typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-23 |
Detector | ADSC CCD |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 270.372, 270.372, 270.372 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 182.570 - 3.990 |
R-factor | 0.265 |
Rwork | 0.262 |
R-free | 0.31000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pem |
RMSD bond length | 0.007 |
RMSD bond angle | 1.036 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | BEAST |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 4.070 |
High resolution limit [Å] | 4.000 | 4.000 |
Rmerge | 0.150 | 0.390 |
Number of reflections | 29196 | |
<I/σ(I)> | 13.5 | 4 |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 15.1 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 1:1 MIX OF PROTEIN SOLUTION (8 MG/ML R1E, 4 MG/ML R2F, 1.5 MM DGTP, 1.5 MM ADP, 1.5 MM MGCL2 AND 1.5 MM HYDROXYUREA) AND RESERVOIR SOLUTION CONSISTING OF 100 MM NAHEPES PH 7.5 AND 1.8 M SODIUM FORMATE. |