2BPH
STRUCTURE OF MURINE DECTIN-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 |
| Unit cell lengths | 57.199, 57.199, 72.996 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.198 |
| Rwork | 0.195 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bpd |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.316 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.120 | 0.740 |
| Number of reflections | 13478 | |
| <I/σ(I)> | 12.8 | 2.1 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 4.2 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M POTASSIUM CHLORIDE, 0.05M SODIUM CACODYLATE PH6.5, 0.1M MAGNESIUM ACETATE, 10% PEG 8000, pH 6.50 |
