2BP6
crystal Structure of pseudomonas aeruginosa lectin (PA-IIL) complexed with a-L-Galactopyranoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.629, 72.790, 54.530 |
| Unit cell angles | 90.00, 94.38, 90.00 |
Refinement procedure
| Resolution | 19.430 - 1.500 |
| R-factor | 0.143 |
| Rwork | 0.143 |
| R-free | 0.16600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uzv |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.398 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.430 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.230 |
| Number of reflections | 63528 | |
| <I/σ(I)> | 10.35 | 3.08 |
| Completeness [%] | 97.0 | 95.1 |
| Redundancy | 3.82 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | TRIS HCL 0.1M, PH8.5, 1.75 M AMMONIUM SULFATE, pH 8.50 |
