2BOZ

Photosynthetic Reaction Center Mutant With Gly M203 Replaced With Leu

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector	ADSC
Spacegroup name	P 31 2 1
Unit cell lengths	138.613, 138.613, 185.274
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.960 - 2.400
R-factor	0.175
Rwork	0.174
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	WILD-TYPE RHODOBACTER SPHAEROIDES UNPUBLISHED DATA
RMSD bond length	0.018
RMSD bond angle	1.881
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.100	0.510
Number of reflections	80240
<I/σ(I)>	10	2
Completeness [%]	99.5	97.6
Redundancy	5.4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1