2BOP
CRYSTAL STRUCTURE AT 1.7 ANGSTROMS OF THE BOVINE PAPILLOMAVIRUS-1 E2 DNA-BINDING DOMAIN BOUND TO ITS DNA TARGET
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Detector technology | AREA DETECTOR |
| Detector | SDMS |
| Spacegroup name | R 3 2 |
| Unit cell lengths | 64.600, 64.600, 64.600 |
| Unit cell angles | 61.80, 61.80, 61.80 |
Refinement procedure
| Resolution | 6.000 - 1.700 |
| R-factor | 0.2007 |
| RMSD bond length | 0.020 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | pH 7.50, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 3350 | ||
| 3 | 1 | 1 | BIS-TRIS-PROPANE_HCL | ||
| 4 | 1 | 1 | NACL | ||
| 5 | 1 | 1 | KCL | ||
| 6 | 1 | 1 | CACL2 | ||
| 7 | 1 | 1 | YBCL3 | ||
| 8 | 1 | 2 | WATER | ||
| 9 | 1 | 2 | PEG 3350 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein-DNA complex | 11.7 (mg/ml) | |
| 10 | 1 | reservoir | 50 (mM) | ||
| 11 | 1 | reservoir | 2 (mM) | ||
| 2 | 1 | drop | Tris-HCl | 28.8 (mM) | |
| 3 | 1 | drop | 75 (mM) | ||
| 4 | 1 | drop | PEG3350 | 9.25 (%) | |
| 5 | 1 | drop | 12.5 (mM) | ||
| 6 | 1 | drop | 0.5 (mM) | ||
| 7 | 1 | drop | lanthanide chloride | 1.5 (mM) | |
| 8 | 1 | reservoir | PEG3350 | 35-37 (%) | |
| 9 | 1 | reservoir | Tris | 40 (mM) |
