2BOO
The crystal structure of Uracil-DNA N-Glycosylase (UNG) from Deinococcus radiodurans.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 63.500, 85.100, 85.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 84.520 - 1.800 |
| R-factor | 0.236 |
| Rwork | 0.234 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1akz |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.841 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.360 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.100 | 0.270 |
| Number of reflections | 40425 | |
| <I/σ(I)> | 4.44 | 2.65 |
| Completeness [%] | 93.7 | 95.2 |
| Redundancy | 2.52 | 2.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 277 | 0.2 M AMMONIUM NITRATE, 17% (W/V) PEG 3000 AT 4 DEGREES C, pH 7.50 |
