2BON
Structure of an Escherichia coli lipid kinase (YegS)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-11-07 |
| Detector | ADSC QUANTUM-4 |
| Wavelength(s) | 0.939, 0.939 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.326, 166.164, 48.474 |
| Unit cell angles | 90.00, 97.03, 90.00 |
Refinement procedure
| Resolution | 83.040 - 1.900 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.26700 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.608 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.300 |
| Number of reflections | 38253 | |
| <I/σ(I)> | 12 | 2.1 |
| Completeness [%] | 97.7 | 96 |
| Redundancy | 2.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 15% PEG 3350 0.1 M BIS-TRIS PH 5.5 0.2 M MGCL2 PLUS 15%PEG400 AS CRYOPROTECTANT. |
