2BOL

CRYSTAL STRUCTURE AND ASSEMBLY OF TSP36, A METAZOAN SMALL HEAT SHOCK PROTEIN

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-20
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	51.590, 102.050, 139.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.020 - 2.500
R-factor	0.207
Rwork	0.207
R-free	0.26400
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	autoSHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.030	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.060	0.180
Number of reflections	25578
<I/σ(I)>	16.9	4.5
Completeness [%]	96.9	81.4
Redundancy	5	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			HANGING DROP 1:1 PROTEIN:RESERVOIR SOLUTION 1.7 M AMMONIUM SULPHATE 10MM DTT 100 MM ADA (PH 6.5) 90 MM SODIUM POTASSIUM TARTRATE