2BOL
CRYSTAL STRUCTURE AND ASSEMBLY OF TSP36, A METAZOAN SMALL HEAT SHOCK PROTEIN
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.590, 102.050, 139.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.020 - 2.500 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.030 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.180 |
| Number of reflections | 25578 | |
| <I/σ(I)> | 16.9 | 4.5 |
| Completeness [%] | 96.9 | 81.4 |
| Redundancy | 5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | HANGING DROP 1:1 PROTEIN:RESERVOIR SOLUTION 1.7 M AMMONIUM SULPHATE 10MM DTT 100 MM ADA (PH 6.5) 90 MM SODIUM POTASSIUM TARTRATE |
