2BOC
Potassium channel KcsA-Fab complex in thallium with tetraethylarsonium (TEAs)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2004-08-08 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 |
| Unit cell lengths | 154.680, 154.680, 76.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.010 |
| R-factor | 0.223 |
| Rwork | 0.222 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4c |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.249 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.110 | 0.430 |
| Number of reflections | 17721 | |
| <I/σ(I)> | 7.03 | 1.83 |
| Completeness [%] | 96.6 | 90.6 |
| Redundancy | 3.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.4 | PEG 400, MAGNESIUM ACETATE, SODIUM ACETATE, pH 5.40 |
