2BO6
DISSECTION OF MANNOSYLGLYCERATE SYNTHASE: AN ARCHETYPAL MANNOSYLTRANSFERASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 150.228, 150.228, 154.285 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.990 - 2.450 |
| R-factor | 0.255 |
| Rwork | 0.253 |
| R-free | 0.27700 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.367 |
| Data reduction software | HKL |
| Data scaling software | HKL |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.990 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.070 | 0.480 |
| Number of reflections | 74145 | |
| <I/σ(I)> | 26 | 4 |
| Completeness [%] | 98.0 | 99 |
| Redundancy | 6.2 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M TRI-SODIUM CITRATE, 0.1 M SODIUM ACETATE TRIHYDRATE, PH 4.6, 30% (V/V) MPD |
