2BO4
Dissection of mannosylglycerate synthase: an archetypal mannosyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 261.148, 151.288, 153.240 |
Unit cell angles | 90.00, 90.41, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.950 |
R-factor | 0.177 |
Rwork | 0.176 |
R-free | 0.19200 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.232 |
Data reduction software | HKL |
Data scaling software | HKL |
Phasing software | SHELX |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.070 | 0.510 |
Number of reflections | 432716 | |
<I/σ(I)> | 15 | 1.6 |
Completeness [%] | 97.0 | 90 |
Redundancy | 3 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.3 M TRI-SODIUM CITRATE, 0.1 M SODIUM ACETATE TRIHYDRATE, PH 4.6, 40% (V/V) MPD |