2BO3
Crystal Structure of HP0242, a Hypothetical Protein from Helicobacter pylori
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL12B2 |
Synchrotron site | SPring-8 |
Beamline | BL12B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-07 |
Detector | ADSC CCD |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 44.300, 44.300, 119.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.720 - 2.270 |
R-factor | 0.254 |
Rwork | 0.254 |
R-free | 0.28600 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.720 | 2.350 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.050 | 0.230 |
Number of reflections | 5957 | |
<I/σ(I)> | 37.2 | 11.9 |
Completeness [%] | 93.6 | 92.4 |
Redundancy | 11.9 | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | pH 8.50 |