2BO3
Crystal Structure of HP0242, a Hypothetical Protein from Helicobacter pylori
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 44.300, 44.300, 119.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.720 - 2.270 |
| R-factor | 0.254 |
| Rwork | 0.254 |
| R-free | 0.28600 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.720 | 2.350 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.050 | 0.230 |
| Number of reflections | 5957 | |
| <I/σ(I)> | 37.2 | 11.9 |
| Completeness [%] | 93.6 | 92.4 |
| Redundancy | 11.9 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | pH 8.50 |
