2BO2
EGF Domains 1,2,5 of human EMR2, a 7-TM immune system molecule, in complex with calcium.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.845, 61.377, 35.584 |
| Unit cell angles | 90.00, 84.10, 90.00 |
Refinement procedure
| Resolution | 29.540 - 2.600 |
| R-factor | 0.239 |
| Rwork | 0.238 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2BOU (BARIUM COMPLEX OF THE SAME PROTEIN) |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.555 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | TNT (5F.0.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.080 | 0.410 |
| Number of reflections | 10840 | |
| <I/σ(I)> | 6.1 | 2.2 |
| Completeness [%] | 99.2 | 99.4 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1-0.175 M CALCIUM ACETATE, 12-16% W/V PEG 8000, 0.1 M NA CACODYLATE BUFFER PH 6.5 |
