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2BO1

Crystal structure of a hybrid ribosomal protein L30e with surface residues from T. celer, and core residues from yeast

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsPAL/PLS BEAMLINE 6B
Synchrotron sitePAL/PLS
Beamline6B
Temperature [K]100
Detector technologyCCD
DetectorBRUKER
Spacegroup nameP 21 21 21
Unit cell lengths29.187, 52.531, 52.692
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution37.190 - 1.700
R-factor0.176
Rwork0.173
R-free0.24000
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1h7m
RMSD bond length0.012
RMSD bond angle1.305
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareCNS
Refinement softwareREFMAC (5.2.0003)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0001.760
High resolution limit [Å]1.7001.700
Rmerge0.0600.090
Number of reflections9277
<I/σ(I)>6334
Completeness [%]98.694.9
Redundancy5.64.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
18.52UL 10MG/ML PROTEIN AND 2UL 22.5% PEG3350, 0.1M TRIS PH 8.5, CRYOPROTECTED IN 20% PEG 400

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PDB entries from 2024-10-30

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