2BNZ
Structural basis for cooperative binding of Ribbon-Helix-Helix Omega repressor to inverted DNA heptad repeats
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.991, 42.505, 103.727 |
Unit cell angles | 90.00, 107.17, 90.00 |
Refinement procedure
Resolution | 100.000 - 2.600 |
R-factor | 0.227 |
Rwork | 0.225 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bnw |
RMSD bond length | 0.012 |
RMSD bond angle | 1.044 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.110 | 0.290 |
Number of reflections | 18516 | |
<I/σ(I)> | 11.7 | 3.1 |
Completeness [%] | 92.8 | 72.2 |
Redundancy | 2.5 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 120 MM NA/KPO4, PH 7.2, 2.2 M DINATRIUMMALONATE, PH 7.5, 3 % 2-METHYL-2,4-PENTANDIOL |