2BNP

Lipidic cubic phase grown reaction centre from Rhodobacter sphaeroides, ground state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-09
Detector	ADSC CCD
Spacegroup name	P 42 21 2
Unit cell lengths	100.142, 100.142, 238.002
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.530 - 2.700
R-factor	0.212
Rwork	0.210
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ogv
RMSD bond length	0.015
RMSD bond angle	2.321
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	120.000	2.790
High resolution limit [Å]	2.700	2.700
Rmerge	0.180	0.790
Number of reflections	32255
<I/σ(I)>	11.4	2.1
Completeness [%]	99.3	100
Redundancy	6.2	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7.5		60% MONOOLEIN/40% 25 MG/ML PROT. (4:1)18% JEFFAMINE M-600, 1M HEPES PH7.5, 0.7M AMM.SULF, pH 7.50