2BNK

The structure of phage phi29 replication organizer protein p16.7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM16
Synchrotron site	ESRF
Beamline	BM16
Temperature [K]	100
Spacegroup name	P 61
Unit cell lengths	95.395, 95.395, 47.572
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.900
R-factor	0.271
Rwork	0.270
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.636
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.970
High resolution limit [Å]	2.900	2.900
Rmerge	0.050	0.170
Number of reflections	5257
<I/σ(I)>	12.3	3.2
Completeness [%]	99.1	99.8
Redundancy	7.2	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1