2BN2

CRYSTAL STRUCTURE OF BOVINE NEUROPHYSIN II COMPLEXED WITH THE VASOPRESSIN ANALOGUE PHE-TYR AMIDE

Replaces:  1BN2

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	289
Detector technology	AREA DETECTOR
Collection date	1989-09-13
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	121.670, 67.960, 62.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.225 *
Rwork	0.208
R-free	0.26200
Structure solution method	SAS
RMSD bond length	0.007
RMSD bond angle	28.500 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	ISAS
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.740
High resolution limit [Å]	2.800 *	2.640
Rmerge	0.058 *
Total number of observations	58983 *
Number of reflections	12840 *
<I/σ(I)>	17.05	3.12
Completeness [%]	83.6	43.45
Redundancy	17

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5 *		5 MG OF PROTEIN WAS DISSOLVED IN 0.5 ML OF WATER, 0.5 MG OF PHENYLALANINE-TYROSINE AMIDE AND 20 MICRO LITERS OF SATURATED AMMONIUM SULPHATE SOLUTION WERE ADDED. THE PH OF THE SOLUTION WAS ADJUSTED TO 6.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate