2BMX
Mycobacterium tuberculosis AhpC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-06-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 139.265, 139.265, 148.525 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 141.420 - 2.400 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.23600 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.819 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.080 | 0.390 |
| Number of reflections | 33798 | |
| <I/σ(I)> | 6.5 | 1.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 31.3 | 31.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M NA CITRATE, PH 6.0, 16% AMMONIUM SULPHATE |
