2BMU
UMP KINASE FROM PYROCOCCUS FURIOSUS COMPLEXED WITH ITS SUBSTRATE UMP AND ITS SUBSTRATE ANALOG AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-03 |
| Detector | MAR-USA, INC |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 144.564, 144.564, 144.564 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.670 - 2.550 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | UNPUBLISHED SEMET PHASED MODEL |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.670 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.090 | 0.390 |
| Number of reflections | 16529 | |
| <I/σ(I)> | 6.8 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.7 | 14.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 3.5 M SODIUM FORMATE, pH 8.00 |
